Melike Aydoğdu

2 papers in the library · 12 citations · publishing 2021-2023

Papers

New Psychoactive Substance 5-MeO-MiPT In vivo Acute Toxicity and Hystotoxicological Study.

Balkan Med J January 1, 2021 Yusuf Ali Altuncı, Melike Aydoğdu, Eda Açıkgöz et al. 10 citations

In mice, a low dose (0.27 mg/kg) of the hallucinogenic tryptamine analog 5-MeO-MiPT did not cause serious histopathological effects on the liver, kidney, or brain. A high dose (2.7 mg/kg) induced apoptotic cell death through caspase activity, with significant increases in Caspase-3 and Caspase-8 positive cells in the liver, kidney, and brain compared to controls. Blood concentrations of 5-MeO-MiPT ranged from 2.7 to 13.4 ng/mL, with higher levels in the liver (15.2-108.3 ng/g) and lower levels in the brain (1.5-40.6 ng/g). The findings suggest that high doses trigger programmed cell death via caspase pathways.

DOI: 10.4274/balkanmedj.galenos.2020.2019.11.68 Full text

Development of a chemometric method in urine sample for newly designed hallucinating psychoactive substances: 5-MeO-MiPT

Ege Tıp Dergisi September 14, 2023 Ezgi Emen, Rukiye Aslan, Melike Aydoğdu et al. 2 citations

A method was developed and optimized for detecting 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) in urine using gas chromatography-mass spectrometry (GC-MS) without derivatization, which shortens preparation time. Chemometric optimization via Plackett-Burman and central composite designs identified sample volume, hydrolysis temperature, and elution volume as the most effective parameters. Optimum conditions were 1 mL urine, 30°C hydrolysis, and 3.5 mL elution volume. The validated method showed linearity from 25-500 ng/mL, a detection limit of 5 ng/mL, quantitation limit of 18 ng/mL, accuracy of 72-101%, and intra-day and inter-day precisions of 4.43% RSD and 4.27% CV, respectively. This approach provides a quick, practical, and accurate tool for analyzing tryptamine analogs.

DOI: 10.19161/etd.1360340 Humanities Art