Development of a chemometric method in urine sample for newly designed hallucinating psychoactive substances: 5-MeO-MiPT

A method was developed and optimized for detecting 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) in urine using gas chromatography-mass spectrometry (GC-MS) without derivatization, which shortens preparation time. Chemometric optimization via Plackett-Burman and central composite designs identified sample volume, hydrolysis temperature, and elution volume as the most effective parameters. Optimum conditions were 1 mL urine, 30°C hydrolysis, and 3.5 mL elution volume. The validated method showed linearity from 25-500 ng/mL, a detection limit of 5 ng/mL, quantitation limit of 18 ng/mL, accuracy of 72-101%, and intra-day and inter-day precisions of 4.43% RSD and 4.27% CV, respectively. This approach provides a quick, practical, and accurate tool for analyzing tryptamine analogs.

Study at a glance

Abstract

Aim: In this study, a method was developed for analysis and chemometric optimization for 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT). Materials and Methods: Our study was carried out in Ege University Institute on Drug Abuse, Toxicology and Pharmaceutical Science, Addiction Toxicology Laboratory. Analysis and optimization of the effects of hydrolysis and solid phase extraction processes during the analysis of 5-MeO-MiPT by Gas Chromatography-Mass Spectrometry (GC-MS). For chemometric screenings design, Plackett-Burman was used. The most effective three factors were determined, and a central composite design was applied, and results were evaluated with surface response methodology. The method was validated for selectivity, linearity, the limit of detection, the limit of quantitation, accuracy, intra-day and inter-day repeatability, stability and carry over. Results: In the chemometric method, the most effective parameters were found sample volume, hydrolysis temperature, and elution volume for 5-MeO-MiPT in urine analysis. Optimum values for these parameters were calculated by surface response methodology and the results were determined 1ml urine volume, 30°C hydrolysis temperature, 3,5 ml elution volume, respectively. The optimized method was validated for selectivity, linearity (25-500 ng/mL), limit of detection (5 ng/mL), limit of quantitation (18 ng/mL), accuracy (72-101%), intra-day and inter-day precisions were measured, respectively (4,43% RSD),(4,27% CV), stability and carry over parameters. Conclusion: With a chemometric approach, quick, practical and accurate method for the detection of 5-MeO-MiPT has been developed with GC-MS. Working of 5-MeO-MiPT without derivatization in GC-MS analysis has shortened the pre-preparation time and is a pioneer for other analogs. It provides an effective method in the analysis of substances such as synthetic analogues from tryptamines which are added every day, with the use of such classical equipment and new methods.