Development of a chemometric method in urine sample for newly designed hallucinating psychoactive substances: 5-MeO-MiPT
Ege Tıp Dergisi September 14, 2023 Ezgi Emen, Rukiye Aslan, Melike Aydoğdu et al. 2 citations
A method was developed and optimized for detecting 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) in urine using gas chromatography-mass spectrometry (GC-MS) without derivatization, which shortens preparation time. Chemometric optimization via Plackett-Burman and central composite designs identified sample volume, hydrolysis temperature, and elution volume as the most effective parameters. Optimum conditions were 1 mL urine, 30°C hydrolysis, and 3.5 mL elution volume. The validated method showed linearity from 25-500 ng/mL, a detection limit of 5 ng/mL, quantitation limit of 18 ng/mL, accuracy of 72-101%, and intra-day and inter-day precisions of 4.43% RSD and 4.27% CV, respectively. This approach provides a quick, practical, and accurate tool for analyzing tryptamine analogs.