UPLC-LTQ-Orbitrap Study on Rat Urinary Metabolites of 5-Methoxy-Alpha-Methyltryptamine
Guo Zhutao, Keran Ding, Shuiqing Zheng, Chunfang Ni, Siyang He, Qian-Ya Deng, Chen Liang
Current Drug Metabolism July 12, 2024 DOI: 10.2174/0113892002295551240628061732 via OpenAlex
Summary
AI-generated from the abstractThe metabolism of the psychedelic drug 5-MeO-AMT was examined in rats. After a single injection, three metabolites appeared in the urine: OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT. The main chemical changes were O-demethylation, hydroxylation of the indole ring, and acetylation of aliphatic amines. These findings help identify and screen for the drug in toxicology and forensic settings.
Study at a glance
| Characteristics | Animal study Peer reviewed |
|---|---|
| Sample size | 5 |
| Population | Rats |
| Intervention | 5-MeO-AMT |
| Dose | 50 mg/kg |
| Duration | 7 days |
| Keywords | Orbitrap Demethylation Urine Hydroxylation High-performance liquid chromatography |
| Key finding | Three metabolites of 5-MeO-AMT were identified in rat urine, with major metabolic pathways involving O-demethylation, hydroxylation, and acetylation. |
Abstract
OBJECTIVE: 5-Methoxy-α-Methyltryptamine (5-MeO-AMT) is a new psychoactive substance which is abused due to its hallucinogenic and euphoric effects. This study aimed to study the metabolic characteristics of 5-MeO-AMT. METHODS: Five rats were given intraperitoneal injection at a dose of 50 mg/kg of 5-MeO-AMT, and their urine was subsequently collected at different times within 7 days. Ultra-high performance liquid chromatographytandem high-resolution mass spectrometry (UPLC-LTQ-Orbitrap) was used to detect the precise molecular weight and fragment ions of 5-MeO-AMT and its possible metabolites in the urine sample extracted with benzene-ethyl acetate. RESULTS: Three metabolites, including OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT were identified in rats' urine. The major metabolic pathways involved O-demethylation, hydroxylation of indole ring, and Acetylation on aliphatic amines. CONCLUSION: The results of this study are an important reference for the identification and screening of toxicants of 5-MeO-AMT.