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Analysis of Psilocybin and Psilocin in Mushroom Extracts by Reversed-Phase High Performance Liquid Chromatography

Bm Thomson

Journal of Forensic Sciences October 1, 1980 DOI: 10.1520/jfs11292j via OpenAlex

Summary

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A method using reversed-phase high performance liquid chromatography separates and measures psilocybin and psilocin in dry and preserved mushrooms. A mobile phase of phosphate buffered methanol, water, and cetrimonium bromide effectively resolves the two hallucinogens.

Study at a glance

Characteristics Method development Peer reviewed
Topics Psilocybin
Keywords Chemistry High-performance liquid chromatography Mushroom poisoning Hallucinogen
Citations 22
Key finding A reversed-phase HPLC method with a phosphate buffered methanol/water/cetrimonium bromide mobile phase separates psilocybin and psilocin in dry and preserved mushrooms.

Abstract

Abstract A method has been developed for the analysis of psilocybin and psilocin in dry and preserved mushrooms using reversed-phase high performance liquid chromatography. A mobile phase of phosphate buffered methanol/water/cetrimonium bromide allows good separation of the two hallucinogens.

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