The Quantitation of Psilocybin in Hallucinogenic Mushrooms Using High Performance Liquid Chromatography
Journal of Forensic Sciences October 1, 1983 DOI: 10.1520/jfs11601j via OpenAlex
Summary
AI-generated from the abstractA method using high-performance liquid chromatography with a specific mobile phase and column enables rapid, selective, and accurate measurement of psilocybin in dried mushroom material. A simple one-step extraction process recovers psilocybin in under 60 minutes. The ratio of absorbance at 267 to 254 nanometers is used to verify the purity of the psilocybin peak.
Study at a glance
| Characteristics | Method development Peer reviewed |
|---|---|
| Topics | Psilocybin |
| Keywords | High-performance liquid chromatography Phosphoric acid Hallucinogen Solid phase extraction |
| Citations | 21 |
| Key finding | A rapid, selective, and accurate HPLC method with a one-step extraction was developed for quantitating psilocybin in dry mushroom material. |
Abstract
Abstract A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The 267:254 nm absorbance ratio is used as a check on peak purity for the psilocybin response.