Distribution of the hallucinogens N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine in rat brain following intraperitoneal injection: application of a new solid-phase extraction LC-APcI-MS-MS-isotope dilution method.
S A Barker, M A Littlefield-Chabaud, C David
Journal of chromatography. B, Biomedical sciences and applications February 10, 2001 DOI: 10.1016/s0378-4347(00)00442-4 via PubMed
Summary
AI-generated from the abstractA method using solid-phase extraction and liquid chromatography-mass spectrometry was developed to measure the indole hallucinogens DMT and 5-methoxy DMT in rat brain tissue. Rats received a 5 mg/kg dose of either compound and were sacrificed after 15 minutes. Brain regions were dissected and analyzed to demonstrate the procedure's applicability. Two new deuterated internal standards were synthesized and used for accurate quantification.
Study at a glance
| Characteristics | Method development and demonstration Peer reviewed |
|---|---|
| Population | Rat brain tissue |
| Interventions | DMT 5-methoxy DMT |
| Dose | 5 mg/kg |
| Keywords | Hallucinogens:DMT:Psychoactive Substances:Psychedelics:Potent Substances:Compounds Neurochemistry:brain chemistry:neuropharmacology:neuroscience |
| Citations | 23 |
| Key finding | The described SPE and LC-APcI-MS-MS-ID method is applicable for detecting and quantifying DMT and 5-methoxy DMT in discrete rat brain regions. |
Abstract
A method for the solid-phase extraction (SPE) and liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric-mass spectrometric-isotope dilution (LC-APcI-MS-MS-ID) analysis of the indole hallucinogens N,N-dimethyltryptamine (DMT) and 5-methoxy DMT (or O-methyl bufotenin, OMB) from rat brain tissue is reported. Rats were administered DMT or OMB by the intraperitoneal route at a dose of 5 mg/kg and sacrificed 15 min post treatment. Brains were dissected into discrete areas and analyzed by the methods described as a demonstration of the procedure's applicability. The synthesis and use of two new deuterated internal standards for these purposes are also reported.