Talanta
January 15, 2008
Bo-Hong Chen, Ju-Tsung Liu, Wen-Xiong Chen et al.
32 citations
Mass spectrometry methods—GC-EI/MS, LC-ESI/MS, and MALDI/TOFMS—were compared for analyzing 13 tryptamine and phenethylamine compounds, including AMT, DMT, 5-MeO-AMT, DET, DPT, DBT, DIPT, 5-MeO-DMT, 5-MeO-DIPT, methamphetamine, 3,4-MDA, 3,4-MDMA, and MBDB. Parent ions were difficult to obtain by GC/MS, but protonated molecular ions were clearly observed by ESI/MS and MALDI/TOFMS. Two major characteristic fragmentations occurred: alpha-cleavage (producing [3-vinylindole]+) and beta-cleavage (producing [CH2N(+)R(N1)R(N2)]). With ESI, alpha-cleavage was the major process; with MALDI, beta-cleavage dominated. The ionization efficiency and fragments from either cleavage depended on the degree of alkylation of the side chain nitrogen.
Journal of chromatography. A
February 15, 2008
Man-Juing Wang, Ju-Tsung Liu, Hung-Ming Chen et al.
12 citations
Nine tryptamines were compared using GC, HPLC, and CE separation techniques. GC/MS and LC/UV-absorption detection limits ranged from 0.5 to 15 µg/mL and 0.3 to 1.0 µg/mL, respectively. CZE/UV-absorption gave detection limits of 0.5–1 µg/mL, while sweeping-MEKC improved detection dramatically to 2–10 ng/mL. Migration times ranged from 11 to 15 minutes by GC, 8 to 23 minutes by HPLC, and 20 to 26 minutes by sweeping-MEKC. The migration order of DMT, DET, DPT, and DBT follows molecular weight, while the order for AMT, 5-MeO-AMT, DIPT, and 5-methoxy-tryptamines can be altered by changing separation conditions.
Applied Sciences
June 22, 2018
Bo-Hong Chen, Ju-Tsung Liu, Hung-Ming Chen et al.
10 citations
Three analytical methods—matrix-assisted laser desorption/time-of-flight mass spectrometry, gas chromatography–electron ionization–mass spectrometry, and liquid chromatography–electrospray ionization/mass spectrometry—were compared for characterizing 20 phenethylamine and tryptamine standards. For GC-EI/MS, three characteristic peaks (the molecular ion and fragments from Cβ-Cα bond breakage) uniquely identified 2C series compounds. For ESI/MS and MALDI/TOFMS, the protonated molecular ion and fragments from α-cleavage and β-cleavage provided useful information for rapid screening and confirmation of phenethylamine and tryptamine derivatives. The assay successfully determined samples containing illicit drugs.