A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry.
Journal of forensic sciences July 1, 2011 Ming J Jin, Changbae Jin, Jin Y Kim et al.
A method using liquid chromatography-tandem mass spectrometry was developed and validated to detect the designer hallucinogen 5-MeO-DIPT and two of its metabolites in rat urine. The technique involved protein precipitation with acetonitrile, chromatographic separation on a C18 column with a methanol-water-formic acid gradient, and multiple-reaction monitoring mass spectrometry. The assay showed a linear range of 0.01-10 μg/mL and a lower limit of quantification of 10 ng/mL for all analytes, with acceptable accuracy and precision. The method was successfully applied to urine from rats given the drug.