Determination of salvinorin A and salvinorin B in Salvia divinorum-related products circulated in Japan.
Kenji Tsujikawa, Kenji Kuwayama, Hajime Miyaguchi, Tatsuyuki Kanamori, Yuko T Iwata, Takemi Yoshida, Hiroyuki Inoue
Forensic science international September 18, 2008 DOI: 10.1016/j.forsciint.2008.07.008 via PubMed
Summary
AI-generated from the abstractSalvinorin A (SalA) and salvinorin B (SalB) were measured in three dried leaf and nine concentrated extract products of Salvia divinorum sold in Japan. SalA content ranged from 3.2 to 5.0 micrograms per milligram in dried leaves and from 4.1 to 38.9 in extracts; SalB ranged from 0.10 to 0.17 in leaves and 0.26 to 2.42 in extracts. The analytical methods used acetonitrile extraction, graphite carbon decolorization, and liquid chromatography-tandem mass spectrometry for confirmation, with quantification by high-performance liquid chromatography for SalA and mass spectrometry for SalB. These data provide a reference for analyzing S. divinorum products on the drug market.
Study at a glance
| Characteristics | Quantitative analysis Peer reviewed |
|---|---|
| Population | Salvia divinorum dried leaf and concentrated extract products circulated in Japan |
| Key finding | Salvinorin A content ranged from 3.2 to 38.9 micrograms per milligram and salvinorin B from 0.10 to 2.42 micrograms per milligram across the products tested. |
Abstract
Two major salvinorins, salvinorin A (SalA) and salvinorin B (SalB), in three Salvia divinorum dried leaf products and nine of its "concentrated extract" products circulated in Japan were determined. These ingredients were extracted twice with acetonitrile and decolored with graphite carbon powder. SalA and SalB were confirmed by liquid chromatography-tandem mass spectrometry in product ion scan mode, and quantified by high-performance liquid chromatography with UV detection (for SalA) and by mass spectrometry in single ion monitoring mode (for SalB). The SalA/SalB contents (mug/mg) were in the range of 3.2-5.0/0.10-0.17 in the dried leaf products and 4.1-38.9/0.26-2.42 in the "concentrated extract" products. These findings would be useful for analysis of S. divinorum-related products circulated in the drug market.