Evaluation of Histopathologic Findings and Safety of Intravitreal Ketamine Administration on Vitreoretinal Tissue in Rat Model: A Pilot Study.
Mehdi Sanatkar, Zohreh Nozarian, Parisa Abdi, Fatemeh Bazvand
Journal of current ophthalmology January 1, 2023 DOI: 10.4103/joco.joco_230_23 via PubMed
Summary
AI-generated from the abstractIntravitreal injection of ketamine at a dose of 0.01 mol/L in rats produced no visible damage to eye tissues. One month after injection, none of the five treated rats showed lens opacity, retinal layer necrosis or atrophy, or optic disc damage. No inflammation was observed in the vitreous, retinal layers, choroid, optic disc, or optic nerve. The same was true for the five control rats that received saline. The findings suggest that this specific dose of intravitreal ketamine is safe for intraocular structures in rats.
Study at a glance
| Characteristics | Controlled preclinical study Pilot study Peer reviewed |
|---|---|
| Sample size | 10 |
| Population | Rats |
| Intervention | Intravitreal injection of ketamine |
| Dose | 0.1 ml of ketamine 0.01 mol/L |
| Duration | 1-month follow-up |
| Topics | Ketamine |
| Keywords | Intravitreal Rat Ophthalmology Drug-safety |
| Key finding | Intravitreal injection of ketamine at 0.01 mol/L caused no adverse histological effects on intraocular tissues in rats. |
Abstract
To evaluate the safety and histological findings of intravitreal injection of ketamine in rats. Each rat received a total volume of 0.1 ml of ketamine 0.01 mol/L (5 rats as ketamine group) or a total of 0.1 ml of normal saline 0.9% (5 rats as control group) under general anesthesia in a sterile condition. A histology assessment was performed 1 month after the intravitreal injection. Lens opacity, necrosis, and atrophy of retinal layers and optic disc were not seen in five specimens in the ketamine group and five in the normal saline group. There was no inflammation in the vitreous, retinal layers, choroid, optic disc, and optic nerve in both groups. Intravitreal injection of ketamine in a special dose has no obvious adverse effect on diverse intraocular tissue.