Micro-solid phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry for the determination of stimulants, hallucinogens, ketamine and phencyclidine in oral fluids
Manuel Sergi, Dario Compagnone, Roberta Curini, G. D’ascenzo, Michele Del Carlo, Sabino Napoletano, Roberta Risoluti
Analytica Chimica Acta July 23, 2010 DOI: 10.1016/j.aca.2010.07.011 via OpenAlex
Summary
AI-generated from the abstractA new method for detecting multiple illicit drugs in biological samples uses a modified fiberglass tip coated with octadecylsilane to extract drugs from the sample before analysis. The method can identify amphetamine, methamphetamine, MDMA, cocaine, benzoylecgonine, ketamine, mescaline, phencyclidine, psilocybine, and others. Quantification is done with liquid chromatography-tandem mass spectrometry. Detection limits range from 0.3 ng/mL for cocaine to 4.9 ng/mL for psilocybine, with all analytes showing coefficients of determination above 0.99, meeting forensic toxicology guidelines.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Topics | Mescaline |
| Keywords | Benzoylecgonine Chemistry Phencyclidine Solid phase extraction |
| Citations | 61 |
| Key finding | A micro-solid phase extraction method using modified fiberglass tips with octadecylsilane can effectively extract and quantify a range of illicit drugs from biological samples with high sensitivity. |
Abstract
A confirmatory method for the determination of illicit drugs based on micro-solid phase extraction with modified tips, made of a functionalized fiberglass with apolar chains of octadecylsilane into monolithic structure, has been developed in this study. Drugs belonging to different chemical classes, such as amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxyethylamphetamine, methylenedioxymethylamphetamine, cocaine, benzoylecgonine, ketamine, mescaline, phencyclidine and psilocybine were analyzed. The quantitation was performed by liquid chromatography-tandem mass spectrometry and the analytes were detected in positive ionization by means of an electrospray source. The limits of quantification ranged between 0.3 ng mL(-1) for cocaine and 4.9 ng mL(-1) for psilocybine, with coefficients of determination (r(2)) >0.99 for all the analytes as recommended in the guidelines of Society of Forensic Toxicologists-American Association Forensic Sciences.