RNA synthesis in isolated brian nuclei after administration of d-lysergic acid diethylamide (LSD) in vivo.
Proceedings of the National Academy of Sciences March 1, 1975 DOI: 10.1073/pnas.72.3.837 via OpenAlex
Summary
AI-generated from the abstractTwo and a half hours after rabbits received LSD intravenously, their isolated brain nuclei showed increased RNA synthesis. Transcription rose by 54% in brain stem nuclei and by 13% in cerebral hemisphere nuclei compared to saline controls. Both nucleoplasmic and nucleolar RNA synthesis were elevated. The primary activity in the assay came from nucleoplasmic RNA polymerase, as alpha-amanitin reduced synthesis by over 70% in both drug and control groups.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Young rabbits |
| Intervention | d-lysergic acid diethylamide (LSD) |
| Duration | 2.5 hr after administration |
| Topics | LSD |
| Keywords | Rna In vivo Transcription linguistics Molecular biology |
| Citations | 24 |
| Key finding | LSD stimulated RNA synthesis in isolated brain nuclei, with a 54% increase in brain stem and 13% in cerebral hemisphere nuclei over controls. |
Abstract
RNA synthesis in isolated brain nuclei was analyzed 2.5 hr after the intravenous administration of d-lysergic acid diethylamide (LSD) to young rabbits. The drug stimulated transcription by 54% in brain stem nuclei and by 13% in cerebral hemisphere nuclei expressed over saline controls. Both nucleoplasmic and nucleolar RNA synthesis were increased. The main activity in the isolated nuclei assay was due to nucleoplasmic RNA polymerase, since alpha-amanitin reduced synthesis by over 70% in either drug or control treatments.