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Determination of psilocin and psilocybin using flow injection analysis with acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection respectively

Nicole Anastos, Neil W. Barnett, Simon W. Lewis, Nicholas Gathergood, Peter J. Scammells, Dorothy A. Sims

Talanta February 17, 2005 DOI: 10.1016/j.talanta.2004.11.038 via OpenAlex

Summary

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A new method using flow injection analysis with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence can detect psilocin and psilocybin at very low concentrations—limits of detection are 9×10⁻¹⁰ M for psilocin and 3×10⁻¹⁰ M for psilocybin. The technique is simple, rapid, and sensitive. The paper also describes a concise three-step synthetic route for psilocin from readily available starting materials, with structures confirmed by spectroscopic data.

Study at a glance

Characteristics Method development Peer reviewed
Topics Psilocybin
Keywords Potassium permanganate Chemiluminescence Ruthenium Tris
Citations 32
Key finding Flow injection analysis with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence provides a sensitive method for detecting psilocin and psilocybin, with detection limits of 9×10⁻¹⁰ M and 3×10⁻¹⁰ M, respectively.

Abstract

A simple, rapid and sensitive method for the determination of psilocin and psilocybin is described. This is the first report on the determination of psilocin and psilocybin using flow injection analysis with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence. The limits of detection (signal-to-noise ratio=3) are 9x10(-10)M and 3x10(-10)M for psilocin and psilocybin, respectively. A concise synthetic route for psilocin in three steps from readily available starting materials is also described. The structures were elucidated on the basis of spectroscopic data.

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