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Psilocybin ameliorates neuropathic pain-like behaviour in mice and facilitates gabapentin-mediated analgesia

Tatum Askey, Daniel Allen-Ross, Daniil Luzyanin, Reena Lasrado, Gary Gilmour, Stephen P. Hunt, Francesco Tamagnini, Maqsood Ahmed, Gary J. Stephens, Maria Maiarù

September 17, 2025 preprint DOI: 10.1101/2025.09.15.676273 via OpenAlex

Summary

AI-generated from the abstract

A single dose of psilocybin produced a sustained anti-nociceptive effect in a mouse model of chronic neuropathic pain, in both male and female mice. This effect was mediated by 5-HT2A receptors, though other mechanisms may also contribute. Psilocybin also significantly increased the anti-nociceptive potential of gabapentin, a common neuropathic pain treatment, suggesting longer-lasting changes in network processing. These findings provide the first preclinical evidence that psilocybin could be a valuable approach for treating chronic pain from nerve injury and serve as a new therapeutic addition for pain management.

Study at a glance

Characteristics Preclinical study
Population Male and female mice with chronic neuropathic pain
Interventions Psilocybin Gabapentin
Dose a single dose
Key finding A single dose of psilocybin produced sustained anti-nociceptive effects in a mouse model of chronic neuropathic pain and enhanced the effect of gabapentin.

Abstract

Abstract Chronic pain states are challenging to control with current drug therapies. Here, we demonstrate that a single dose of psilocybin can produce a sustained anti-nociceptive effect in a model of chronic neuropathic pain in male and female mice. Psilocybin anti-nociceptive effects were mediated by 5-HT 2A receptors, although additional mechanisms might also be involved. Furthermore, a single dose of psilocybin caused a significant increase in the anti-nociceptive potential of gabapentin, a widely used treatment for neuropathic pain consistent with the establishment of longer lasting changes in network processing. Overall, these findings present the first preclinical evidence that psilocybin could be a valuable approach for treating chronic pain from nerve injury and serve as a new therapeutic addition for pain management.

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