A draft sequence reference of the Psilocybe cubensis genome
Kevin Mckernan, Liam T. Kane, Seth Crawford, Chen-Shan Chin, Aaron Trippe, Stephen Mclaughlin
F1000Research April 9, 2021 DOI: 10.12688/f1000research.51613.1 via OpenAlex
Summary
AI-generated from the abstractUsing high-fidelity single molecule sequencing, the genome of the psychoactive Psilocybe cubensis mushroom was assembled. The genome is 46.6 megabases in size, has a guanine-cytosine content of 46%, and is organized into 32 contigs with an N50 of 3.3 megabases. Completeness assessment shows 97.6% of conserved orthologs are present, with 1.2% duplicated. The psilocybin synthesis cluster is located on a single 3.2 megabase contig. The assembled genome sequence is publicly available through NCBI BioProject under accessions PRJNA687911 and PRJNA700437.
Study at a glance
| Characteristics | Genome assembly Peer reviewed |
|---|---|
| Population | Psilocybe cubensis mushroom |
| Keywords | Contig Computational biology Whole genome sequencing Genetics Alkaloids |
| Citations | 3 |
| Key finding | The genome of Psilocybe cubensis is 46.6 Mb, 46% GC, assembled into 32 contigs with an N50 of 3.3 Mb, and the psilocybin synthesis cluster resides on a single contig. |
Abstract
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.