Unraveling the metabolomic profile and acute toxicity of ethanolic extract from Mimosa tenuiflora (Willd.) Poir. root bark.
Renato Pinto De Sousa, Carlos Mario Freitas De Oliveira, Rita de Cassia De Lima Sousa, Lucas Luan Lima Leite, André Luiz Ozório Oliveira, Jady Vitoria Barjud Pereira Ferreira, Ana Marcia De Freitas Pessoa, Jorge Luiz Silva Oliveira, Victor de Jesus Silva Meireles, Esmeralda Maria Lustosa Barros, Adriana Maria Viana Nunes, Mariana Helena Chaves, Maurício Pires de Moura Do Amaral, Gerardo Magela Vieira Júnior
Toxicon : official journal of the International Society on Toxinology October 1, 2024 DOI: 10.1016/j.toxicon.2024.108076 via PubMed
Summary
AI-generated from the abstractAn ethanolic extract of Mimosa tenuiflora root bark, used in a psychedelic drink called 'jurema wine' in Indigenous Brazilian rituals, was analyzed for chemical composition and acute oral toxicity. Thirteen compounds were identified, including proanthocyanidins, alkaloids, a flavonol, and a dihydrochalcone. In female Swiss mice, single oral doses of 300 and 2000 mg/kg caused no deaths, no changes in food intake or body weight, and the lethal dose 50 (LD50) exceeded 2000 mg/kg, placing the extract in GHS category 5. However, both doses significantly altered relative kidney weights, hematological and biochemical parameters, and caused histopathological changes in the heart, liver, and kidneys, indicating acute oral toxicity.
Study at a glance
| Characteristics | Acute oral toxicity study Peer reviewed |
|---|---|
| Population | Female Swiss mice |
| Dose | 300 and 2000 mg/kg |
| Duration | 14 days |
| Keywords | Acute toxicity Chemical profile Mimosa tenuiflora Oecd 423 Root bark |
| Citations | 6 |
| Key finding | The ethanolic extract of Mimosa tenuiflora root bark had an LD50 greater than 2000 mg/kg but caused significant changes in kidney weights, hematological and biochemical parameters, and histopathological alterations in the heart, liver, and kidneys. |
Abstract
Mimosa tenuiflora (Fabaceae) is popularly known in Brazil as "Jurema preta". From the bark of its root, "jurema wine" is obtained, a psychedelic drink used in Indigenous religious rituals in Northeastern Brazil. This work aimed to investigate the chemical composition and acute oral toxicity of the ethanolic extract of the root bark from M. tenuiflora (EEMt). EEMt was analyzed by UPLC-QToF-MS/MS and DI-ESI-IT-MSn. Oral administration of EEMt was performed once at doses of 300 and 2000 mg/kg in female Swiss mice. Signs and symptoms of intoxication, as well as mortality were monitored for 14 days. Thirteen compounds were annotated in EEMt: eight type B proanthocyanidins, three alkaloids, a glycosylated flavonol, and a dihydrochalcone derivative. The acute administration of 300 and 2000 mg/kg of EEMt did not show mortality. It also did not change the food intake or body weight of the animals. However, the relative weights of the kidneys were significantly changed for both doses. Changes in hematological and biochemical parameters were found. In addition, histopathological changes were also observed in the heart, liver, and kidneys. Thus, based on our findings, EEMt presented an LD50 greater than 2000 mg/kg and was therefore classified in category 5 of the Globally Harmonized Classification System (GHS). EEMt showed acute oral toxicity by altering hematological, biochemical and histological parameters.