Gas chromatographic-mass spectroscopic characterisation of the psychotomimetic indolealkylamines and their in vivo metabolites.
B R Sitaram, L Lockett, M Mcleish, Y Hayasaka, G L Blackman, W R Mcleod
Journal of chromatography November 27, 1987 DOI: 10.1016/0378-4347(87)80435-8 via PubMed
Summary
AI-generated from the abstractA new method combining liquid chromatography with gas chromatography-mass spectrometry provides unequivocal verification of structurally characteristic metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine. Previously, metabolite identity was confirmed using multiple analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. The described approach enables separation, characterization, and quantitation of metabolites formed both in vitro and in vivo.
Study at a glance
| Characteristics | Methodological paper Peer reviewed |
|---|---|
| Citations | 9 |
| Key finding | Combining liquid chromatography with gas chromatography-mass spectrometry allows unequivocal verification of structurally characteristic metabolites of N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine. |
Abstract
The use of liquid chromatography with on-line fluorescence detection has formed the basis for the separation, characterisation and quantitation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine formed both in vitro and in vivo. Verification of the identity of metabolites has previously been facilitated by the combined use of a number of analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. We now describe the combination of liquid chromatography with gas chromatography-mass spectrometry for the unequivocal verification of a number of structurally characteristic metabolites of the psychotomimetic indolealkylamines.