Identification of 2,5-dimethoxy-4-ethylthiophenethylamine and its metabolites in the urine of rats by gas chromatography-mass spectrometry.
Li-Chan Lin, Ju-Tsung Liu, Shiu-Huey Chou, Cheng-Huang Lin
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences December 25, 2003 DOI: 10.1016/j.jchromb.2003.09.052 via PubMed
Summary
AI-generated from the abstractA gas chromatography-mass spectrometry method identified four metabolites of the psychoactive compound 2C-T-2 in rat urine after a single 20 mg/kg dose. The metabolites included 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol, 4-ethylthio-2,5-dimethoxyphenyl acetic acid, and two isomeric acetamido metabolites. Based on these findings, a metabolic pathway for 2C-T-2 in rats is proposed.
Study at a glance
| Characteristics | In vivo study Peer reviewed |
|---|---|
| Sample size | 3 |
| Population | Male rats |
| Intervention | 2C-T-2 |
| Dose | 20 mg/kg |
| Duration | 24 hours post-administration |
| Keywords | Biotransformation Metabolic pathway Compound breakdown Drug metabolism Pharmacokinetics |
| Citations | 29 |
| Key finding | Four metabolites of 2C-T-2 were identified in rat urine, leading to a proposed metabolic pathway for the compound. |
Abstract
A simple and specific method based on gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS) for the analysis of in vivo metabolism of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) in rats is described. Three male rats were administered 20 mg/kg of 2C-T-2 by intra-peritoneal injection, and 24 h urine fractions were collected before and after the administration for analysis. After acidic hydrolysis of the urine samples, the metabolites were liquid-liquid extraction and analyzed by a quadruple mass spectrometer in the selected ion monitoring mode. The findings show that four metabolites of 2-(4-ethylthio-2,5-dimethoxyphenyl)-ethanol (Mw: 242), 4-ethylthio-2,5-dimethoxyphenyl acetic acid (Mw: 256), 1-acetoamino-2-(2-hydroxy-4-ethylthio-5-methoxyphenyl)-ethane (Mw: 269) and 1-acetoamino-2-(2-methoxy-4-ethylthio-5-hydroxyphenyl)-ethane (Mw: 269) are present and the metabolic pathway for 2C-T-2 in the rat is proposed.