[Authentication and ultra performance liquid chromatography (UPLC)/MS analysis of magic mint, Salvia divinorum and its related plants].
Takuro Maruyama, Hiroyuki Kamakura, Ruri Kikura-Hanajiri, Yukihiro Goda
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan January 1, 2008 DOI: 10.1248/yakushi.128.179 via PubMed
Summary
AI-generated from the abstractBefore Salvia divinorum was regulated under Japan's Pharmaceutical Affairs Law, commercial Salvia cultivars sold in Japan were tested for the hallucinogen salvinorin A. Ultra performance liquid chromatography/mass spectrometry showed that none of the cultivars contained salvinorin A, whereas S. divinorum leaves and its processed product "concentrated salvia" contained 0.19% to 0.58% of the compound. A DNA-based authentication method using amplification refractory mutation system clearly distinguished S. divinorum from the cultivars. The authors conclude that this authentication method is simple and accurate, making it useful for practical regulation.
Study at a glance
| Characteristics | Analytical study Peer reviewed |
|---|---|
| Population | Commercial Salvia cultivars available in the Japanese market and Salvia divinorum leaves and processed products |
| Topics | Salvia divinorum |
| Keywords | Magic mint Psychoactive plant Hallucinogenic plant Plant identification |
| Citations | 14 |
| Key finding | None of the commercial Salvia cultivars contained salvinorin A, while S. divinorum leaves and its processed products contained 0.19% to 0.58% of the compound. |
Abstract
Ultra performance liquid chromatography (UPLC)/mass spectrometry (MS) analysis was performed to investigate whether commercial Salvia cultivars available in the Japanese market contain salvinorin A (1), which is an hallucinogen present in magic mint (Salvia divinorum) prior to the regulation of S. divinorum by the Japanese Pharmaceutical Affairs Law. In addition, a previously reported method to authenticate S. divinorum, utilizing an amplification refractory mutation system (ARMS) was applied to the same samples to estimate the method's accuracy. As a result of the UPLC/MS analysis, it was clear that none of the tested cultivars possessed 1 while S. divinorum leaves and its processed products "concentrated salvia" contained 1 in the range from 0.19% to 0.58%. Furthermore, the ARMS method could clearly distinguish S. divinorum from the tested cultivars. In conclusion, the authentication method is considered to be useful for the practical regulation of S. divinorum due to its simplicity and accuracy.