Psilocybin was isolated from the mushroom Psilocybe argentipes and its presence was determined in specimens of several mushroom species. The work details the chemical isolation and analytical methods used to identify and quantify the alkaloid in these fungi.
Magic mushrooms sold in Japanese markets are difficult to identify by appearance alone, especially when dried and powdered. By analyzing the internal transcribed spacer region of their ribosomal RNA gene, researchers classified the samples into six genetic groups and matched them to known species using DNA databases. Chemical analysis showed that Panaeolus cyanescens contained the highest level of psilocin among the mushrooms tested, while Amanita species contained none.
Since 2002, psilocybin-containing fungi have been regulated in Japan, but fly agaric-related products are now entering the Japanese market via the internet. DNA analysis of these products suggested they originate from Amanita muscaria or A. muscaria var. persicina, and they were classified into three strains based on nucleotide sequences. Chemical analysis detected harmine derivatives and/or tryptamine derivatives in some products, and the matK gene of Peganum harmala was found in all harmine-containing samples, indicating the presence of additional psychoactive plants.